Chemical Studies and Antibacterial Activity of the Root of Rumex abyssinicus
One of the Ethiopian endemic plants used traditionally as medicine for different diseases is Rumex abyssinicus. It is widely known as ‘Meqmeqo’ in Amharic. The aim of this study was to isolate and characterize compounds from the root of R. abyssinicus and to test their bioactivities of solvent fractions of the extract. The crude extract was extracted from dried powdered root of the plant with methanol. Then it was fractionated with n‑hexane and ethyl acetate with increasing polarity. The yellow powder obtained from ethyl acetate fractionation was applied to column chromatographic separation that led to the isolation of two compounds, diisobutyl phthalate and emodine. Finally, antibacterial evaluation of the ethyl acetate and methanol soluble portions were carried out against four pathogenic bacteria. Both methanol and ethyl acetate soluble portions inhibited all selected bacterial in better at high concentrations.
W. Abebe, An overview of Ethiopian traditional medicinal plants used for cancer treatment, European Journal of Medicinal Plants 14(4) (2016), 1-16. https://doi.org/10.9734/EJMP/2016/25670
A. Altemimi, N. Lakhssassi, A. Baharlouei, D. G. Watson and D. A. Lightfoot, Phytochemical: Extraction, isolation, and identification of bioactive compounds from plant extracts, Plants 6 (2017), 42. https://doi.org/10.3390/plants6040042
T. T. Ayele, M. B. Regasa and D. A. Delesa, Antibacterial and antagonistic activity of selected traditional medicinal plants and herbs from East Wollega Zone against clinical isolated human pathogens, Science, Technology and Arts Research Journal 4(3) (2015), 175-179. https://doi.org/10.4314/star.v4i3.26
T. Teklehaymanot, M. Giday, G. Medhin and Y. Mekonnen, Knowledge and use of medicinal plants by people around Debre Libanos monastery in Ethiopia, Journal of Ethnopharmacology 111(2) (2007), 271-283. https://doi.org/10.1016/j.jep.2006.11.019
B. Abera, Medicinal plants used in traditional medicine by Oromo people, Ghimbi District, Southwest Ethiopia, J. Ethnobiology Ethnomedicine 10 (2014), Article No. 40. https://doi.org/10.1186/1746-4269-10-40
F. Fullas, A Short Note on Meqmeqo (Rumex abyssinicus), November 22, 2015. Accessed Available: http://www.ethiomedia.com/aa2nov15/4491.html
N. Worku, A. Mossie, A. Stich, A. Daugschies, S. Trettner, N. Y. A. Hemdan and G. Birkenmeier, Evaluation of the in vitro efficacy of Artemisia annua, Rumex abyssinicus, and Catha edulis Forsk extracts in cancer and Trypanosoma brucei cells, International Scholarly Research Notices 2013, Art. ID 910308, 10 pp. https://doi.org/10.1155/2013/910308
F. Melkamu, R. Padmanabhan and G. Gurmessa, Phytochemical investigation and in vitro antibacterial evaluation on root extracts of Rumex abyssinicus, Nat. Prod. Chem. Res. 4(6) (2016), 239. https://doi.org/10.4172/2329-6836.1000239
B. S. Joseph, P. H. Kumbhare and M. C. Kale, Preliminary phytochemical screening of selected medicinal plants, Int. Res. J. of Science & Engineering 1(2) (2013), 55-62.
G. O. de Silva, A. T. Abeysundara and M. M. W. Aponso, Extraction methods, qualitative and quantitative techniques for screening of phytochemical from plants, American Journal of Essential Oils and Natural Products 5(2) (2017), 29-32.
K. Sahira Banu and L. Cathrine, General techniques involved in phytochemical analysis, International Journal of Advanced Research in Chemical Science 2(4) (2015), 25-32.
A. Z. Yusuf, A. Zakir, Z. Shemau, M. Abdullahi and S. A. Halima, Phytochemical analysis of the methanol leaves extract of Paullinia pinnata linn, J. Pharmacognosy Phytother. 6(2) (2014), 10-16. https://doi.org/10.5897/JPP2013.0299
M. L. Hossain, M. A. A. S. M Hossain, K. K. Sarkar, A. Hossain and M. A. Rahman, Phytochemical screening and the evaluation of the antioxidant, total phenolic content and analgesic properties of the plant Pandnus foetidus (family pandanaceae), Int. Res. J. Pharmacy 4(2) (2013), 170-172.
C. J. Semenya, R. B. Maseko and S. S. Gololo, Comparative qualitative phytochemical analysis of the different parts of Barleria dinteri (Oberm): A contribution to sustainable use of the plant species, Journal of Pharmaceutical, Chemical and Biological Sciences 6(2) (2018), 52-59.
G. Jayapriya and F. G. Shoba, Screening for phytochemical activity of Urechites lutea plant, Asian Journal of Plant Science and Research 4(6) (2014), 20-24.
N. Morsy, Phytochemical analysis of biologically active constituents of medicinal plants, Main Group Chemistry 13 (2014), 7-21. https://doi.org/10.3233/MGC-130117
E. Abebe and N. Mekonnen, In Vitro antibacterial activity of rumex nervosus, plantago lanceolata, solanum incanum and lepidium sativum against selected bacterial pathogens of human and animals, Ethiopian Veterinary Journal 20(2) (2016), 119-131.
Y. Besufekad, S. Beri, T. Adugnaw and K. Beyene, Antibacterial activity of Ethiopian Lepidium sativum L. against pathogenic bacteria, Journal of Medicinal Plants Research 12(6) (2018), 64-68. https://doi.org/10.5897/JMPR2017.6321
L. Abate and B. Ayalew, Assessment of total content of secondary metabolites, in vitro free radical scavenging potential and peroxide value on different solvent extracts of Rumex abyssinicus Jacq. root, Journal of Chemical and Pharmaceutical Research 11(2) (2019), 39-46.
E. Mulisa, K. Asres and E. Engidawork, Evaluation of wound healing and anti-inflammatory activity of the rhizomes of Rumex abyssinicus J. (Polygonaceae) in mice, BMC Complement Altern. Med. 15 (2015), 341. https://doi.org/10.1186/s12906-015-0878-y
R. W. Bussmann, P. Swartzinsky, A. Worede and P. Evangelista, Plant use in Odo-Bulu and Demaro, Bale region, Ethiopia, J. Ethnobiology Ethnomedicine 7 (2011), 28. https://doi.org/10.1186/1746-4269-7-28
F. A. Awe, A. O. Giwa-Ajeniya, A. A. Akinyemi and G. N. O. Ezeri, Phytochemical analysis of Acalypha wilkesiana, Leucaena leucocephala, Peperomia pellucida and Senaa alata Leaves, The International Journal of Engineering and Science 2(9) (2013), 41-44.
T. V. Dougnon, J. R. Klotoé, J. Sègbo, J.-M. Atègbo, A. P. Edorh, F. Gbaguidi, A. S. Hounkpatin, C. Dandjesso, L. Fah, B. Fanou, K. Dramane and F. Loko, Evaluation of the phytochemical and hemostatic potential of J atropha multifida sap, African Journal of Pharmacy and Pharmacology 6(26) (2012), 1943-1948. https://doi.org/10.5897/AJPP12.351
G. Alam, M. P. Singh and A. Singh, Wound healing potential of some medicinal plants, International Journal of Pharmaceutical Sciences Review and Research 9(1) (2011), 136-145.
G. Zelalem and D. Dula, Isolation, characterization and antibacterial activities evaluation of Rumex abyssinicus rootbark extracts, Nat. Prod. Chem. Res. 7(1) (2019), 353. https://doi.org/10.4172/2329-6836.1000353
G. Challachew, Phytochemical investigation and antibacterial activity of the root of Rumex abyssinicus, M.Sc. Thesis, Wollo University, 2019.
A. I. Husein, M. S. Ali-Shtayeh, R. M. Jamous, W. J. Jondi and N. Abd-Aljapar Zatar, Phthalate derivatives are naturally occurring in Arum Palaestinum, Int. J. Curr. Res. Aca. Rev. 2(9) (2014), 195-203.
This work is licensed under a Creative Commons Attribution 4.0 International License.