GC-MS analysis of Mirabilis japala L. root extract and studying its antimicrobial and antioxidant activity
Abstract
Mirabilis jalapa L., a tropical plant widely grown as a medicinal plant worldwide, was studied for the antimicrobial and antioxidant activities of its root extract. Methanolic (MMRE) and aqueous (AMRE) extracts of Mirabilis jalapa L. root were prepared and tested at concentrations of 2000, 4000, and 6000 ppm for antimicrobial activity against Salmonella spp., Bacillus subtilis, Pseudomonas aeruginosa, Escherichia coli (E. coli), Leuconostoc spp., Bacillus thuringiensis, Aspergillus niger, Penicillium spp., and Rhizopus oryzae. Both MMRE and AMRE exhibited inhibitory activity against the tested organisms, with inhibition zone diameters increasing with higher extract concentrations. The highest inhibition zones at 6000 ppm of MMRE were 19.3 mm and 41.7 mm against Bacillus subtilis and Aspergillus niger, respectively. GC–MS analysis was used to identify the bioactive compounds in MMRE and AMRE. Antioxidant capacity was evaluated based on the DPPH free radical scavenging activity of the extracts. Among the tested concentrations, 250 mg/ml of MMRE and AMRE showed the highest scavenging activities of 96% and 89%, respectively.
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